Structure of cloned DNA complementary to rat prolactin messenger RNA.

Primary sequence of ovomucoid messenger RNA as determined from cloned complementary DNA

Ovomucoid messenger RNA (mRNAom) comprises approximately 8% of the total mRNA in the estrogen-stimulated oviduct. The recombinant plasmid pOM100 contained DNA complementary to the 3' end of mRNAom. DNA complementary to the 5' end of mRNAom was obtained from a partially purified preparation of mRNAom by polymerization by reverse transcriptase in the presence of a restriction fragment primer from...

Nucleotide sequence of cloned rat serum albumin messenger RNA.

The nucleotide sequences of the recombinant DNA inserts of three bacterial plasmid clones containing nearly all of the rat serum albumin mRNA have been determined. A statistical analysis of the nucleotide sequence reveals a pattern of repeated internal homology that confirms the "intragenic triplication" model of albumin evolution.

Quantitation of rat alpha-fetoprotein messenger RNA with a complementary DNA probe.

Rat a,-fetoprotein (AFP), an estrogen-binding protein secreted by fetal liver and many fast growing hepatomas, is barely detectable in normal adult animals. As part of a study of the mechanisms controlling the expression of the AFP gene, we have compared the levels of rnRN&r,, in normal liver and in hepatomas by RNA-cDNA hybridization techniques. cu,-Fetoprotein messenger RNA was isolated by im...

Synthesis of DNA complementary to 14S calf lens crystallin messenger RNA by reverse transcriptase.

Resistance of Cloned 1F5 Chimeric Anti-CD20 Antibody Heavy-Chain Gene to DNA Polymerase due to a Predicted Hairpin Structure

Background: Formation of secondary structure such as DNA hairpins or loops may influence molecular genetics methods and PCR based approaches necessary for genetic engineering, in addition to gene regulation. Materials and Methods: A polymerase chain reaction with splice overlap extension (SOE-PCR) was used to create fully synthetic 1F5 chimeric anti-CD20 heavy- and light-chain genes. The chi...